Journal: Microbiology Spectrum

Article Title: Targeted metagenomic assessment reflects critical colonization in battlefield injuries

doi: 10.1128/spectrum.02520-23

Figure Lengend Snippet: Microbial detection following targeted capture sequencing of samples containing a defined polymicrobial pathogen control genomic DNA mix at 100,000 copies

Article Snippet: The polymicrobial skin commensals standard (ATCC MSA-1005) comprised Acinetobacter johnsonii, Corynebacterium striatum, Micrococcus luteus, Cutibacterium acnes, Staphylococcus epidermidis, and Streptococcus mitis .

Techniques: Sequencing, Control

Journal: Microbiology Spectrum

Article Title: Targeted metagenomic assessment reflects critical colonization in battlefield injuries

doi: 10.1128/spectrum.02520-23

Figure Lengend Snippet: Microbial detection following targeted capture sequencing of samples containing a defined polymicrobial skin commensal control genomic DNA mix at 10,000 copies

Article Snippet: The polymicrobial skin commensals standard (ATCC MSA-1005) comprised Acinetobacter johnsonii, Corynebacterium striatum, Micrococcus luteus, Cutibacterium acnes, Staphylococcus epidermidis, and Streptococcus mitis .

Techniques: Sequencing, Control

Journal: Microbiology Spectrum

Article Title: Targeted metagenomic assessment reflects critical colonization in battlefield injuries

doi: 10.1128/spectrum.02520-23

Figure Lengend Snippet: Detection of virulence genes via targeted sequencing capture panel in single and polymicrobial control reference genomes ^a

Article Snippet: The polymicrobial skin commensals standard (ATCC MSA-1005) comprised Acinetobacter johnsonii, Corynebacterium striatum, Micrococcus luteus, Cutibacterium acnes, Staphylococcus epidermidis, and Streptococcus mitis .

Techniques: Sequencing, Control

Journal: Microbiology Spectrum

Article Title: Targeted metagenomic assessment reflects critical colonization in battlefield injuries

doi: 10.1128/spectrum.02520-23

Figure Lengend Snippet: Detection of virulence and AMR genes via targeted sequencing capture panel in polymicrobial control reference gDNA. Detection of off-reference (genes that are detected by the panel but not present in silico ) and undetected (genes that are present in silico but are not detected by the panel) virulence factor genes in (A) skin polymicrobial mixture spike-in reported as log 2 (read counts). Each row represents a virulence gene and black-colored tiles indicate expected detection in silico . Detection of AMR genes in gDNA of (C) skin and (D) pathogenic polymicrobial mixture spike-ins reported as normalized read counts. Detection results were compared to expected genes based on reference sequences. Results are shown at coverage thresholds of 50%, 75%, and 100% and are represented by green-, yellow-, and blue-colored tiles, respectively, while-black colored tiles indicate expected detection in silico . All expected and detected genes are listed with each row representing an AMR gene.

Article Snippet: The polymicrobial skin commensals standard (ATCC MSA-1005) comprised Acinetobacter johnsonii, Corynebacterium striatum, Micrococcus luteus, Cutibacterium acnes, Staphylococcus epidermidis, and Streptococcus mitis .

Techniques: Sequencing, Control, In Silico